Elvis' hair is black during the final "Love Me Tender" reprise, but closer to blonde in the rest of the film.
When the brothers try to coerce the other former soldiers into turning in the money, they disarm the three. As the camera angles switch, one guy has his jacket covering his holster, then it is exposed with the gun still in it, then switches back to being concealed.
The Civil War did not end all at once on April 9, 1865. The surrender and ceasefire on that date applied only to General Robert E. Lee's Confederate Army of Northern Virginia and the Virginia theater of operations. The other large Confederate army, General Joseph E. Johnston's Army of Tennessee, did not surrender until April 26, and smaller elements of Confederates throughout the country (which were incommunicado, such as the cavalry brigade that the Reno brothers belonged to) continued to fight until the news of Johnston's surrender reached them through the slow communications of the day, well into the summer of 1865. (Whilst that fact would not necessarily prevent the charges of robbery leveled by the Federal Government against the Reno brothers, it would make a compelling legal defense for them.)
When Clint goes outside to check on a noise and finds the other soldiers there, the chambers of his gun are empty.
A Confederate soldier zips up the pants that were taken off Northern soldiers, before zippers were invented.
In the scene where Cathy Reno is at the window crying, a car can be seen in the background.
Vance takes a Smith & Wesson New Model No. 3. from Mr. Siringo, which was not released until 1878.
During the robbery of the payroll train, up the track in the direction of the train's travel, there are modern CTC block signals of the Southern Pacific Railroad which were installed in the 1930s (beyond the siding switch). Centralized Traffic Control for railroads did not exist in 1865, nor did the heavier rail on the right of way shown in the film (at least 100 pounds).
When the locomotive engineer is ordered to halt his train, he applies an air brake. The air brake system was designed and patented by George Westinghouse on April 13, 1869.
Elvis takes his hands off his guitar during "We're Gonna Move", but the sound of the guitar can still be heard.