This was the first American film to be shot in Romania, particularly in the aftermath of the fall of Communism in Romania.
Radu is named after Radu The Handsome, brother of Romanian prince Vlad The Impaler, who himself was the inspiration for the quintessential vampire Count Dracula.
The Subspecies minions were originally going to be played by live actors in costume. Director Ted Nicolaou thought that the stuntmen were over-acting and that it didn't look well overall. Nicolaou then brought on FX artist David W. Allen to work with the subspecies stop-motion puppets.
The song "Nightwing" by Swedish black metal band Marduk from their 1998 album of the same name is a cover of the main theme of Subspecies, and the song itself is about the main antagonist Radu Vladislas.
Elements of the film, particularly the Subspeices minions, are actually recycled from an unmade film called "The Subterraneans", which in turn, was originally to have been filmed in the 1980s by producer Charles Band's previous studio, Empire International, in Rome.