During the game's opening of the Bravo Team, Rebecca's pistol has a flashlight attachment on it. During the game however, the flashlight attachment disappears completely.
Billy's handcuffs while in-game are gold, but in the fully rendered FMV's the handcuffs are silver.
In the shot where Rebecca first approaches the Ecliptic Express, there is not a drop of rain in the scene. Seconds into her approach, in the next two shots, rain is shown suddenly falling as though it had been raining for minutes.
The information for the Hookshot device states the weight capacity in kilograms. The setting is in the United States of America, which uses pounds.
When the crows first appear, the windows in the room break before the crows actually burst through.
Some of the writing on the S.T.A.R.S. badge is upside-down.
When Rebecca looks at the B.O.W. in suspended animation on the screen, the scrolling text misspells the word "humanoid".
During the game, the train travels a considerable distance, which at the speed it is going put the train dozens of miles away from Raccoon City by the time it stops.