To play as Mary Jane, type in the code girlnextdoor. In later versions of the game, the code was removed (it only works in the older copies). It was removed because in the last level, Mary Jane and Spider-Man are supposed to kiss and when typing in the code girlnextdoor, you remain as Mary Jane and two Mary Jane's are kissing.
As an homage to Sam Raimi, Bruce Campbell, Raimi's high school friend and cinematography associate, has his voice featured as the narrator for all of the Spider-Man games as well as having a cameo in all of Raimi's Spider-Man movies.
If you beat the game on Hero Difficulty, you get to play as Harry Osborn as the Green Goblin, complete with glider and various explosive devices.
Kraven the Hunter only appears in the Xbox version of the game.
This is the first Spider-Man game to be published on the Xbox.