This episode was originally meant to be the first episode, but it aired second.
This episode was supposed to be the first one to air because it was produced first, but because Cartoon Network often airs episodes out of production order, it aired second.
This is the first episode of the series where the girls was seen inside in school.
During Nap Time in Powerpuff Bluff, one of the other sleeping kids is Dexter from Dexter's Laboratory.
First appearance of Ms. Keane in the episode "Powerpuff Bluff".