When Anne was in her world, it appeared before leaving the class while at school in the lab she was dissecting a frog that looks like Hop Pop.
According to an storyboard, Sasha was originally supposed to bleed during her fight with Anne.
During a shot of Anne's school, three students resembling the main characters of the "Earthbound" videogames (Paula, Jeff, and Ness) can be glimpsed.
Among the objects in Sasha's room is a hat closely resemebling Mickey Mouse's iconic sorcerer hat from the film "Fantasia" (1940).
The science teacher in Anne's frog-dissecting class has a "Science!" logo on her shirt. This same logo appears on a sign on the wall in the secret science lab under the Plantar's house in "Family Shrug."