The mushroom kid that first sees the humans starts the rumor of "aliens" being nearby. In the shot where he tells Hip and Hop, the color of his outfit and the spots on his hat are darker. And his outfit turns into a one piece instead of overalls and a shirt like he was wearing.
In one shot when Hip and Hop set up the mirror to make it look like there's no wall, Hop's eyes are green and not white.
When Kooky is passing the rumor about aliens to Big Mouth, Kooky's neck is missing and his hair is showing where his neck should be.
Mario and Luigi help two ladies up after they fall. When Luigi asks if anyone has seen the "aliens," Mario's mustache is brown.
In the first shot of Kooky analyzing the family's brainwaves with his invention, his face is brown.
Koopa asks his kids who started the rumor that there were aliens. When the kids all point fingers and say "he did," their mouths open and their lips don't move.