As Luigi is trying to look behind Kootie Pie when she's human to see if there's really a tail, he corners her to the stairway. In the shot of her at the stairway before striking Luigi, her tail is white instead of brown.
When Kootie Pie puts her face to the barred window in the dungeon door, her lower face is brown. In the next shot, it's back to her regular white color.
In the close up of Cheatsy reacting as Luigi is transformed back to his human self while kissing Kootie Pie, Cheatsy's head is orange instead of green.
When the Boomerang Brothers are attempting to attack the Mario Brothers, they change colors from orange to green in between shots.
In the shot where Koopa orders the Fire Chomps to attack the Mario Brothers, Cheatsy's head is orange instead of green and his eye lids are black instead of green.
While Kootie Pie is struggling with the wrench on the oil pipes, she changes colors from bright to dark as she's moving.
At the shot of Toadstool, Toad, and the Sultan were leaving the dungeon cell, Kootie Pie Koopa's head is clearly seen detached from her body.
As Kootie Pie said, "Daddy only wants me to be happy," her mouth didn't move.