The muffins that Applejack made in this episode would not cause food poisoning to humans if properly baked.
Derpy Hooves, the fan-favorite pegasus with crazy eyes, can be briefly seen in the public at 04:45 and 06:01, as well as at the 14:52 mark in the "Muffins!" crowd.
Friendship Report: Applejack learned that it is just as okay to accept help as it is to give help.
In this episode, Big Macintosh talks in full sentences, but in most of the series, he usually says "Eeyup" and "Nope".