Twilight seems to have gotten much better at teleporting. In previous episodes, teleporting only once seemed to exhaust her, but she teleports several times in this episode without showing any signs of fatigue.
Starting this episode, the magic aura glows of Twilight, Rarity and Celestia's horns have changed; Twilight's aura has changed from light purple to hot pink, Rarity's has changed from pale blue to luminous sky blue, and Celestia's has changed from aqua to gold.
Derpy Hooves, the fan-favorite pegasus with crazy eyes, can be seen briefly in the train at 00:44 during the intro, flying above the crowd at 17:05 and 17:55 and finally fighting with the Mayor over the plush at 17:55.
The music being played while Celestia confronts Twilight and her friends is similar to the score "The King's Speech," from the film of the same name.
Friendship Report: Twilight and her friends learned that they should take a friend's worries seriously, even if you feel that it is not something to worry about.