Airplane! (1980)
"Surely, you can't be serious?
- I am serious, and don't call me 'Shirley'!"
The exchange shouldn't exceed two sentences, otherwise we're not talking about quotes but about dialogue, so sorry for the Pulp Fiction (1994) fans but the iconic "What" sequence between Jules and Brett is ineligible for this poll.
Want to discuss it? -It's here my friend."